Chromatography Institute of America > Standard Courses > HPLC Maintenance & Troubleshooting Course
 
Topics

Learn:

  • Principles of separation and system/component operation
  • Techniques for rapidly determining the problem and how to solve it
  • How to identify and troubleshoot chromatography problems including: resolution, baseline, equilibration, precision, sensitivity,carry-over, mobile phase, retention time problems, and many more
  • How to deal with peak problems, such as: tailing, fronting, split peaks, negative peaks, shoulder peaks, broad peaks, ghost peaks,stepped peaks, contaminant peaks, and more
  • Troubleshooting techniques for physical problems: high and low pressure, leaks, tubing and connections issues, gradient problems,sample vial problems, and much more
  • Proper ways to store and clean the system and columns
  • How to identify and fix typical hardware problems: pump seals,solenoid valves and check valves, autosampler leaks and probe wash solutions, detector lamp and dirty flow cells

 

Click here for a Course Syllabus 

Chapters
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