Chromatography Institute of America > Standard Courses > HPLC Analysis and Purification of Proteins and Peptides Course


  • Fundamentals of ion exchange, hydrophobic interaction, size exclusion/gel filtration, and reversed phase HPLC techniques for proteins and peptides
  • Three major "tools" for improving separations
  • Practical method optimization techniques
  • How to choose solvents and buffers
  • About narrow bore vs high speed columns
  • Scaling up for preparative separations
  • How to optimize gradient separations
  • System troubleshooting
  • The latest techniques in sample preparation
  • Integration and ways to optimize for accuracy


Click here for a Course Syllabus 

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