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HPLC Analysis and Purification of Proteins and Peptides

Celebrating 20 Years of training excellence!

Benefits

High performance Liquid Chromatography has become one of the most important tools of the protein/peptide chemist in recent years. The need to isolate proteins and peptides in sufficient quantities to identify activity and determine structure, as well as to analyze for purity, has placed heavy demands on laboratory personnel to become proficient in the use of this technique. Individuals working in Biological Research, Biotechnology, Proteomics, Pharmaceutical Quality Control, Clinical Investigation, Foods/Feeds Analysis, and related areas, who want to gain a better understanding of, and improve their skills in HPLC, will find no better course than this.

This new and totally up-to-date course is noncommercial (no sales pitches like some other courses), practical, and enjoyable. It is geared for beginning to intermediate levels of HPLC experience. Those just beginning in HPLC will gain a solid understanding of the basics, and will obtain a much greater grasp of how to use the technology to operate successfully with a minimum of downtime. Those with greater familiarity will advance in their efficiency and ease with HPLC. They will learn to develop and modify methods, and gain in their overall understanding of the variables, resulting in greater success and higher productivity.

Topics
Learn:
  • Fundamentals of ion exchange, hydrophobic interaction, size exclusion/gel filtration, and reversed phase HPLC techniques for proteins and peptides
  • Three major "tools" for improving separations
  • Practical method optimization techniques
  • How to choose solvents and buffers
  • About narrow bore vs high speed columns
  • Scaling up for preparative separations
  • How to optimize gradient separations
  • System troubleshooting
  • The latest techniques in sample preparation
  • Integration and ways to optimize for accuracy
Course Section Titles:
  • Introduction to HPLC of Proteins & Peptides
  • HPLC Theory
  • Mobile Phases
  • Ion Exchange
  • Reversed Phase
  • Size Exclusion
  • Hydrophobic Interaction
  • Sample Preparation
  • Gradient HPLC
  • Detection/Quantitation
  • Troubleshooting LC Systems

Each person will receive a course notebook containing copies of all graphics and materials used in the course.

Program Agenda:

The course will run each day from 8:30A.M. to 4:45 P.M. Lunch is included.

The Schedule for
HPLC Analysis and Purification of Proteins and Peptides Course







Northeast Region
Boston,MADirectionsDec. 10-11, 2009
Boston, MA
King of Prussia,PADirectionsMar 25-26, 2010
King of Prussia, PA
Waltham,MADirectionsOct. 14-15, 2010
Waltham, MA
North Central Region
Deerfield,ILDirectionsJun. 17-18, 2010
Deerfield, IL
Middle Atlantic Region
Research Triangle,NCDirectionsApr. 22-23, 2010
Research Triangle, NC
Rockville,MDDirectionsSept 23-24, 2010
Rockville, MD
South Atlantic Region
South Central Region
Irving,TXDirectionsFeb 11-12, 2010
Irving, TX
Houston,TXDirectionsDec. 9-10, 2010
Houston, TX
Western Region
San Diego,CADirectionsNov. 12-13, 2009
San Diego, CA
Irvine,CADirectionsJan 21-22, 2010
Irvine, CA
Burlingame,CADirectionsMay 20-21, 2010
Burlingame, CA
Seattle,WADirectionsJuly 22-23, 2010
Seattle, WA
Broomfield,CODirectionsAug 19-20, 2010
Broomfield, CO
Berkeley,CADirectionsNov. 18-19, 2010
Berkeley, CA
Europe
Copenhagen,DKDirectionsOn Site Course
Customer SiteCopenhagen, DK

How to Register

Click on location above to register on line
or
Click here to view the printable form.

Print form and indicate method of payment (check, purchase order, or credit card).

The tuition fee for this two-day course is $850 per person. If three or more register at the same time, the tuition fee is reduced to $800 per person, Save $50 each, with our multi-person discount!

Save even more by combining this course with the new HPLC Maintenance & Troubleshooting Course. If registered seperately, the cost would be $1,300, but by registering for both courses together, the tuition is only $1,250. If three or more register at the same time, the tuition fee is further reduced to $1,200 per person. A savings of $100 per person!

Cancellations can be made without penalty up to one week preceding the course. Later cancellations are subject to a cancellation charge.

Class Size is Limited!
Register Now!

Chromatography Institute of America
HPLC Training Dept.
2816 Hiwall Court
Castle Rock, CO 80109
Toll Free in the US: (800)788-9922 or
Local Phone: (303)471-8015
Fax: (303)471-8025
E-mail: CIA@HPLCinstitute.com